The Memory Display contents might or might not change but they should look similar to the following picture. You are now ready to send the image to your DPU-550 so press the Send File button where the red arrow points.
You might get a warning dialog asking if you want to unlock the involved lock region(s). Press the Yes button since it is required in order to program the DPU-550.
SAM-BA will now send the binary image down to the DPU-550 which should only take a few seconds. It will then ask if you want to lock the involved lock region(s). Press the No button since there is no reason to lock them.